The last phase of spermatogenesis involves profound morphological and functional changes that are essential for male fertility: elongation of the cell, cytoplasm reduction and acquisition of new structures such as the acrosome and the flagellum. Importantly, during this step, the spermatid nucleus is compacted to ~one fifth of its original size and spermatid chromatin is extensively remodeled. Spermatid chromatin remodeling is essential to properly compact the paternal genome and preserve its integrity until fecundation. The regulation of all these processes requires a genetic program unique to postmeiotic male germ cells, with thousands of genes only expressed at that time, a significant proportion of those genes are located on the X and Y chromosomes. Abnormal (epi)genetic regulation usually leads to male infertility, and could also have an impact on the progeny, when male infertility is bypassed using Assisted Reproductive Technology. The project aims at studying genetic/epigenetic regulators and their dynamics during the differentiation of male gametes using mouse as a model and single-cell transcriptomic analyses.
We invite applications from highly motivated and qualified individuals holding, or shortly expecting to be awarded, a PhD degree in molecular and cellular biology, epigenetics or genomics. The candidates will have proven research skills in those fields attested by a track record of research/academic achievement and publications in internationally recognized peer-reviewed journals. Prior experience with NGS (genomics or transcriptomics) and good knowledge of bioinformatic and statistical analyses are essential. An experience in single-cell transcriptome analyses will be an advantage. Good communication skills (in English) and team spirit are also indispensable. Only applications from junior candidates (i.e. with < 3 years of postdoctoral experience) with the above-described profile will be considered.
Our group is part of the Team «Genomics, Epigenetics and Physiopathology of Reproduction», which studies the molecular bases of reproductive disorders through a multidisciplinary approach. We are part of the « Development, Reproduction & Cancer Department » of the Institut Cochin, one of the largest French Biomedical Research Center which consists of 38 research teams and 10 state-of-the-art core facilities. The group and host institute, located in central Paris, will provide a dynamic and interactive environment.
Institut Cochin website: www.institutcochin.fr
Application Deadline: 31/03/2018
Début/Beginning: June 2018
Durée du contrat/Length of contract: 2 years with possible extension
Structure employeur/organization: INSERM
Applicants should send their CV (including publication record), letter of motivation and name of 2 references to Julie Cocquet. Only apply if your profile matches the position description.
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